https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18545 Wed 11 Apr 2018 12:52:16 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32347 Wed 09 Mar 2022 15:59:14 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18247 Sat 24 Mar 2018 08:04:50 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29660 CYP2R1, CYP27B1 and CYP24A1) and the Vitamin D receptor gene (VDR). This analysis was conducted in the context of dietary Vitamin D, and background methyl donor related biochemistry, with adjustment for several dietary and lifestyle variables. Percentage methylation at CpG sites was assessed in peripheral blood cells using methylation sensitive and dependent enzymes and qPCR. Standard analytical techniques were used to determine plasma 25(OH)D and homocysteine, and serum folate and B12, with the relationship to methylation status assessed using multi-variable regression analysis. CYP2R1 and VDR methylation were found to be independent predictors of plasma 25(OH)D, when adjusted for Vitamin D intake and other lifestyle variables. CYP24A1 was related to plasma 25(OH)D directly, but not in the context of Vitamin D intake. Methyl-group donor biochemistry was associated with the methylation status of some genes, but did not alter the relationship between methylation and plasma 25(OH)D. Modulation of methylation status of CYP2R1, CYP24A1 and VDR in response to plasma 25(OH)D may be part of feedback loops involved in maintaining Vitamin D homeostasis, and may explain a portion of the variance in plasma 25(OH)D levels in response to intake and sun exposure. Methyl-group donor biochemistry, while a potential independent modulator, did not alter this effect.]]> Mon 26 Apr 2021 10:02:52 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40176 Fri 22 Jul 2022 13:54:23 AEST ]]>